571306	4734	56164	110811	121097	PIG53	-	NEDD4	STK31	NEDD4-1|RPF1	SGK396|TDRD8	Biochemical Activity	physical	Persaud A (2009)	19953087	9606	9606	High Throughput	-	Ubiquitination	-	High Throughput: A ubiquitination assay was applied to a protein microarray in order to identify substrates of the E3 enzyme Nedd4.  The reaction contained E1,  UbcH5b [E2], and FITC-labelled ubiquitin.|High Throughput: Only those proteins that showed four independent interactions were considered substrates (i.e. significant signals found for proteins printed in duplicate on two separate microarrays).  The top ~50 hits were selected as high-confidence data sets.	-	BIOGRID